BIOT 6650 — Polymerase Chain Reaction Techniques and Assay Optimization
1 semester hoursGraduateLecture
Focuses on the practical execution and optimization of polymerase chain reaction used in research, biopharmaceutical, diagnostic, and quality control environments. Emphasizes hands-on practice with PCR workflows including primer design considerations, reaction assembly, reagent and buffer selection, and thermal cycling parameters. Highlights the relationship between assay conditions and product outcomes through gel-based evaluation of amplification quality. Reinforces reproducibility and industry-aligned workflow standards through rigorous documentation and analytical recordkeeping.